Journal: Molecular Medicine Reports
Article Title: CXCL1 gene silencing inhibits HGC803 cell migration and invasion and acts as an independent prognostic factor for poor survival in gastric cancer
doi: 10.3892/mmr.2016.5843
Figure Lengend Snippet: CXCL1 gene silencing inhibited HGC803 cell invasion. Representative images of the invaded cells (stained with hematoxylin) selected from three experiments are presented above. Migrated cells were counted under a microscope. CXCL1, chemokine (C-X-C motif) ligand 1; siRNA, small interfering RNA.
Article Snippet: The cells were incubated with rabbit anti-human CXCL1 monoclonal antibody (1:3,000 dilution; cat. no. sc-2778; Santa Cruz Biotechnology, Inc.) and mouse anti-human D2-40 monoclonal antibody (1:3,000 dilution; cat. no. 182410; Invitrogen; Thermo Fisher Scientifc, Inc.) at 37°C for 2 h. Content determination results were analyzed by HMIAS-2000 automatic medical color image analysis system (Qianping Image Technology Co., Ltd., Wuhan, China).
Techniques: Staining, Microscopy, Small Interfering RNA